6x Xylene Cyanol Loading Buffer for Electrophoresis

This buffer will have only the light blue than runs slower on the gel. This protocol will make 40ml of 6x solution. Adjust to your needs if necessary.

1. 100mg Xylene-cyanol
2. 6g of Ficoll (Type 400)
3. Add water to 40 ml

Dissolve well. It takes a while. An option is to shake at 37 degrees overnight.

6x Bromophenol Blue Loading Buffer for Electrophoresis

This protocol will make 2 liter of 50x solution. Adjust to your needs if necessary.

This buffer will have only the dark blue than runs faster on the gel. This protocol will make 40ml of 6x solution. Adjust to your needs if necessary.

1. 100mg Bromophenol Blue
2. 6g of Ficoll (Type 400)
3. Add water to 40 ml

Dissolve well. It takes a while. An option is to shake at 37 degrees overnight.