Important: Label tubes with buffer name, date, your initials, and tube number

10x PCR buffer (see Promega PCR buffer) for 20 ml

500 mM KCl (10 ml 1M stock)

100 mM Tris/HCl (pH 9.0) (2 ml 1M stock)

15 mM MgCl₂ (0.3 ml 1M stock)

1% Triton X (1 ml 10% Triton X)

Add sterile MilliQ up to 20 ml in total.

Note 1: Renew 10xPCR buffer every 1~2 months.

Taq polymerase

1 (current ('07) stock Taq) : 9 (storage buffer)

MgCl₂ (25 mM)

Add 250 µl 1M MgCl₂ to 10 ml dH₂O

300 µl aliquots in 0.5 ml tubes.

dNTP (100mM total, 25 mM each)

Add equal amount of dATP, dTTP, dCTP, dGTP (100 mM, Fermentas dNTP Set #R0186)

Make 100 µl aliquots.