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Room 2115
Section of Plant Biology
1002 Life Sciences, One Shields Ave.
University of California Davis
Davis, CA 95616


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Hypocotyl measurement with ImageJ


  1. ImageJ (from
  2. Working scanner (can be hard)

Scanner Settings

  1. Set to Grayscale.
  2. Set to 600 ppi.
  3. Set to wtb points.
  4. Set to Custom ~130,255.
  5. Set Preferences -> Scan Material -> Positive.
  6. Set Preferences -> More -> Scan Quality -> Quality.
  7. Set Preferences -> More -> Interpolation -> Speed.

Image J

  1. Go to Analyze -> Set Scale.
  2. Over there change:

Distance in Pixels -> 600

Known Distance -> 25.4

Pixel Aspect Ratio -> 1

Unit of Length -> mm